03000cz a2200301n 4500 sh2003004761 DLC 20250804134902.3 030626i| anannbabn |a ana c sh2003004761 (DLC)5958139 (DLC)sh2003004761 (DLC)301928 WaU eng DLC Surface plasmon resonance Plasmon resonance, Surface Resonance, Surface plasmon Sensing, Surface plasmon resonance SPR (Surface plasmon resonance) Surface plasmon resonance sensing g Biosensors g Optical detectors g Plasmons (Physics) Work cat.: Shumaker-Parry, J.S. Quantitative aspects of SPR spectroscopy and SPR microscopy, applications in protein binding to immobilized vesicles and dsDNA arrays, 2002: p. 1 (Surface plasmon resonance sensing has emerged as an important technique for monitoring the adsorption of biomolecules from liquid solutions onto functionalized solid surfaces ... As a biosensing technique, SPR can be used to detect refractive index changes ...) MESH browser, May 21, 2003 (Surface Plasmon Resonance. A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.) WWW, May 21, 2003 (Surface plasmon resonance is a phenomenon which occurs when light is reflected off thin metal films. A fraction of the light energy incident at a sharply defined angle can interact with the delocalized electrons in the metal film (plasmon) thus reducing the reflected light intensity. The precise angle of incidence at which this occurs is determined by a number of factors, ... [the principal factor is] the refractive index close to the backside of the metal film, to which target molecules are immobilized and addressed by ligands in a mobile phase running along a flow cell. If binding occurs to the immobilized target the local refractive index changes, leading to a change in SPR angle, which can be monitored in real-time by detecting changes in the intensity of the reflected light, producing a sensorgram) Inspect, via WebSPIRS, May 22, 2003 (surface plasmon resonance; SPR; surface plasmon resonance sensing) AccessScience dictionary, via WWW, May 22, 2003