| LC control no. | sh2014000740 |
|---|---|
| LC classification | QP519.9.V65 Biochemistry |
| Topical heading | Voltage-sensitive dyes |
| Variant(s) | Potentiometric dyes Potentiometric probes VSDs (Voltage-sensitive dyes) |
| See also | Dyes and dyeing Fluorescent probes Potentiometry--Equipment and supplies |
| Found in | Work cat: Bachtel, A.D. A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di 4 ANEPPS using pulsed LED excitation, 2010: p. 1 (In cardiac and neuronal tissue, for example, rapid changes in Vm [transmembrane potential] are represented as action potentials (AP), which have a distinct morphology that can be recorded using voltage sensitive dyes (VSD), a group of fluorophores that exhibit changes in fluorescence with changes in Vm) Membrane potential imaging in the nervous system, 2010: p. 125 (Voltage-sensitive dyes, or potentiometric probes, are molecular voltmeters that insert into, but do not cross cell membranes, where they intercalate among the phospholipids that compose either leaflet of the bilayer. There, by mechanisms that are not entirely understood, they sense a representative portion of the membrane electric field and alter their light absorption and/or emission in response to changes in that field, thereby providing the physical basis for optical measurement of membrane potential) p. 13 (More recently, we have synthesized voltage-sensitive dyes (VSDs) with new hemicyanine chromophores that have absorbance and emission further toward the red end of the visible spectrum. ... There are several other labs that are currently actively working on synthesizing new potentiometric dyes) Salzberg, B.M. Functional imaging of nervous systems using voltage-sensitive dyes, via WWW, Mar. 27, 2014: abtr. (Voltage-sensitive dyes are small molecules that bind to cell membranes and change their optical properties, absorption or fluorescence, in response to changes in membrane potential, the voltage across the membrane. These optical changes are extremely rapid and tend to be linear reporters of local electrical events. Thus, these "potentiometric probes" behave as molecular voltmeters and can be used, with a high-speed camera or other image dissector, for functional imaging of electrical activity including action potentials, synaptic potentials, and passive (electrotonic) signals) Journal of membrane biology, Dec. 2003: p. 105 (Interpretation and optimization of absorbance and fluorescence signals from voltage-sensitive dyes. ... Voltage-sensitive dyes produce absorbance and fluorescence changes that can be used to image voltage. The present study develops a systematic approach to the optimization of these signals. ... Voltage-sensitive dyes provide an optical signal suitable for imaging voltage in living tissue. These dyes serve as powerful tools in the study of electrical activity in the nervous system as well as in other electrically excitable tissue) Scripta medica, Dec. 2006: p. 280 (Optical mapping is also widely employed in cardiac electrophysiology animal experiments. The principle of optical mapping is the application of voltage-sensitive dye (VSD) to the examined tissue where it binds to a membrane of cardiac cells. The dye undergoes changes in its fluorescence spectra, in response to changes in the surrounding electrical field. The absorption and fluorescence spectra of the dye are highly dependent on its environment. In general, the dyes are essentially non-fluorescent in water and become quite strongly fluorescent upon binding to membranes. The tissue is illuminated by light with relatively limited narrow spectra. Then, the dye emits fluorescent light of higher wavelength and amplitude proportional to the potential at heart surface. The emitted light can be easily detected and recorded) |
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